DOI: 10.36347/sjams.2023.v11i08.032

Pages: 1572-1576

Introduction: The rate of occurrence of endophthalmitis after cataract surgery has been reduced to a greater extent nowadays. Even then endophthalmitis related consequences are devastating. Several sources of infection, including contamination by air, solutions, surgical instruments, intraocular lenses, and wound leakage have been identified. Aim of the Study: The study aimed to evaluate the influence of two methods of surgical technique of cataract surgery in bacterial as well as comparison of contamination by these two techniques. Methods: This prospective observational study was conducted over 60 patients with age-related cataracts in the Department of Ophthalmology and Microbiology in Sir Salimullah Medical College and Mitford Hospital, Dhaka, Bangladesh, from 1st January 2009 to 30 June 2009. Patients were selected purposively based on specific selection criteria. Selected patients underwent detailed ophthalmic and systemic evaluation as well as relevant investigations. Cataract extraction followed by intraocular lens implantation was planned for all patients. The two techniques of cataract surgery such as SICS and Phacoemulsification were assigned to the patients randomly on a 1:1 basis. 1 ml of anterior chamber contents were aspirated through an aseptic technique by 26G needle from each patient pre- operatively, after capsulorhexis (early per-operative), and just before wound closure by stromal hydration (late per- operative). All samples were sent for 10% KOH staining and culture and sensitivity test after proper leveling. Results: Micro-biological examination shows no sample was positive for 10% KOH staining. In the SICS group, out of 30 samples, 3 were found culture positive, which were positive in 4 and 5 samples in early per-operative and late per-operative samples respectively, and in the Phacoemulsification group, it was 2, 3, 5 pre-operative, early per- operative and late per-operative sample respectively. The common organisms isolated were ......