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Scholars Journal of Agriculture and Veterinary Sciences | Volume-2 | Issue-05
Effect of Fresh Citrus limon Juice on Liver Histomorphology of Growing Rabbits (Oryctolagus cuniculus)
I.P. Solomon, Oyebadejo S.A, Otoho E. A, Joseph O. S
Published: May 30, 2015 | 247 161
DOI: 10.36347/sjavs.2015.v02i05.004
Pages: 347-351
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Abstract
Histomorphology of the Citrus limon was investigated, Twenty four (24) rabbits of mixed breeds aged between 10 – 16 weeks, weighing between 600 – 2185g were used in this experiment to determine the morphological effect of feeding varying dietary levels of citrus limon juice on the histomorphology of the liver of growing rabbits. The rabbits were randomly allocated into four treatments containing 0% (control), 25%, 50% and 75% citrus limon juice administration respectively. Feed and water were served ad-libitum throughout the 5 weeks experimental period. At the end of the juice administration, Final body weight of the animal were taken, animals were sacrificed using chloroform inhalation in a desiccator, liver tissues were extracted, weighed and placed into Neutral buffered formalin, sent to the laboratory for histopathological analysis Changes in Liver weight was not significant (P<0.05) compared to the Body weight while the weight gain in the treatment grups was extremely significant (P<0.001). The histopathological results revealed no cellular abnormality in the entire treatment groups as compared to control group. In conclusion, it is shown that Citrus limon juice can be included up to 75% in rabbit diets without any adverse or deleterious effect on the histomorphology of the liver of rabbits, as it enhances the cyto-architecture thereby preserving and increasing the cellular profile.