DOI: 10.36347/sjavs.2019.v06i06.001

Pages: 160–164

This study was conducted to evaluate the use of swim up methods in vitro fertilization on buffalo oocytes. Buffalo epididymis cauda was sexed with 3 sexing treatments P1 (1 hour), P2 (2 hours) and P3 (3 hours) in Co2 incubator 5%. Oocyte fertilized in vitro with Cauda Epididymal Sperm in each treatment. The results showed that the fertilization rate of cauda epididymal sperm after swim up showed very significant differences (P <0.01) in P2 treatment with a 2-hour swim-up incubation duration in Co2 incubator 5% with a value of 95,222 ± 3.71%. 2 cell division rates showed very significant differences (P <0.01) in P2 treatment with a value of 88.97 ± 5.54 and not significantly different in the development of 8 cells (P> 0.05). It can be concluded that the swim up method has an effect on the level of fertilization and development of oocytes to 2 cells and there has been a decrease in development at the level of 8 cells in buffalo oocytes in vitro.