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Scholars Journal of Agriculture and Veterinary Sciences | Volume-6 | Issue-11
Isolation and Characterization of Total Coliform Bacteria from Drinking Water of District Shangla, Khyberpakhtunkhwa, Pakistan
Muhammad Sohail, Mehwish Malik, Hamidullah, Muhammad Sajid, Muhammad Shoaib, Shazia Mansoor Qureshi, Shafiqurehman
Published: Dec. 9, 2019 | 252 148
DOI: 10.36347/sjavs.2019.v06i11.001
Pages: 262-267
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Abstract
The current study was carried out in district Shangla of Khyber Pakhtunkhwa, Pakistan. A 100 ml of water sample was collected in sterilized bottles and labelled and shifted to Microbiology lab at Veterinary Research & Disease Investigation Center Abbottabad for further analyses. Water samples (n=252) from three different sources viz; spring, Water storage tanks and water supply lines) (n=84) water samples from each source were processed. About 69 samples (27.38%) were found positive for total coliform bacteria and 183 samples (72.62%) were negative. In spring water study, 9 samples (10.71%) were found positive and 75 (89.29%) were negative, whereas from storage water tanks 30 samples (35.72%) were positive and 54 (64.28%) were negative for the total coliform bacteria. From 84 tape water samples, 30 (35.72%) were positive and 54 (64.28%) were negative for the total coliform bacteria. The Colony Forming Unit (CFU) from Tape and storage tank water samples was more than 300 while Escherichia coli was absent in spring water samples. Moreover, positive samples of spring water differ significantly (p>0.0382) from water storage tank (p<0.1104) and tap water (p<0.866). The spring water samples were found more suitable for drinking purpose than tap water and storage tank samples. Most sites met WHO recommended standard microbiological parameters. To certify well health for the people, proper disinfection should be adopted through chlorination or other appropriate methods to provide drinking water.