DOI: 10.36347/sjams.2020.v08i03.039

Pages: 1004-1012

Introduction: Systemic lupus erythematosus is characterized by various autoantibodies which prevalence and clinical significance vary among populations. The aim of our research is to study the immunological profile of autoantibodies in Moroccan population with lupus. Patients and methods: Seventy-seven patients with lupus meeting at least four criteria of the 1997 ACR had an ANA screening by indirect immunofluorescence method (IIF) on HEp-2 substrate (Kallstad, Biorad, threshold = 1: 160), followed by the identification of specific anti-DNAn antibodies ( Aeskulisa, threshold: 16 IU / ml), anti-SSA, SSB, Sm, RNP, Nucleosomes, Histones [ELISA (ENA profile, Biorad) Immuno-Dot (D-Tek, Aesku)] and anti-phospholipid (APL-ELISA, DRG threshold: 10 IU / ml). Results: The mean age of the patients was 37.1 ± 14.13 with female predominance (Sex ratio M / F: 15.7). The clinical manifestations of SLE were dominated by rheumatological (80.6%), dermatologic (76.1%), renal (58.2%), respiratory (34.3%) neurological (28.3%) and cardiac (26.7%) symptoms. The ANA were found in all patients, anti-DNAn in 74.6%, associated with anti-nucleosome Ab and anti-histone in 58.5 and 36.5% of cases respectively. The SSA, Sm, RNP and SSB specificities were noted in 47.8; 37.3; 32.8% and 26.9% of cases respectively, and 19.4% of cases had Antiphospholipids Abs. A statistically significant association was established between anti-DNAn, anti-Sm and anti-RNP with renal impairment (p = 0.0007), pleurisy (p = 0.033) and Raynaud's phenomenon (p = 0.022) respectively. Conclusion: The data in our series show a particularly high level of anti-DNAn Ab and anti-SSA, with a correlation of anti-Sm Ab with pleurisy and anti-RNP with Raynaud's phenomenon. These results underline the interest of these markers in the clinico-immunological characterization of SLE.