DOI: 10.36347/sjams.2018.v06i09.008

Pages: 3264-3268

Acute diarrhoeal illness is common in tropical regions during summer and monsoon seasons microbial agents causing diarrhea can be bacteria, virus, parasite or fungus. Our aim was to study the microbiological agents involved in causing diarrhoea in patients admitted at tertiary hospital Telanagana region.The study group included stool samples of 360 patients admitted in government general Hospital Nizamabad, Telangana suffering with acute diarrhea and stool samples of 50 non diarrhoeal patients as control. The samples were immediately transported to the laboratory and microscopy, culture was done .The identification of the organism was confirmed after biochemical reactions and agglutination tests with the polyvalent sera. Antibiotic susceptibility was done using Kirby Bauer disc diffusion method according to CLSI guidelines phage typing was done at National Institute of Cholera and Enteric disease Kolkata. Among 360 feacal samples, majority collected in summer season included males between 21-40 yrs of age, presented with watery diarrhea (162.) – 45%, (108) –30%bloody diarrhea, (90) -25% with mucus the following organisms were isolated, Bacteria (303)-84.2%; parasite (102)-28.3 % and fungus (09)- 2.5% ; vibrio cholera 66(18.3%) was major isolate followed by E.coli 60 (18%), Campylobacter 12(3.3% ), Shigella flexneri 10(2.7%), Staphylococcus 4(1.1%) Salmonella paratyphi 4 (1.1%). Antibiogram was done which showed 100% sensitivity to amikacin, gentamycin, ciprofloxacin, tetracycline, doxycycline, Cefotaxim and norfloxacin; 100% resistance to cotrimoxazole. Phage typing of vibriocholera done and found to be T-7, T-26.The study shows bacterial isolates were in majority including vibrio cholera ,males of age group 21-40 yrs more involved during summer season.