DOI: 10.36347/sjams.2018.v06i09.046

Pages: 3476-3482

Hepatitis B virus (HBV) is the most common potentially life threatening chronic viral infection affecting millions worldwide. More than 240 million people have chronic HBV liver infections. (serum Alanine amino transferase (ALT) level is the most commonly aced variable for assessment of liver disease) High genetic variability is a characteristic feature of the HBV as the viral polymerase lacks proof reading.Genotyping of HBV is essential for characterization of patients groups and for epidemiological studies. Typical chronic hepatitis B is marked by the presence HBsAg and high levels of HBV DNA with variable elevation of ALT and histological activity.The study was done to study in patient attendance OPD of SMIH Dehradun to study prevalence of various genotypic variants, their association with ALT levels and estimation of HBV viral load in various genotypes. A total of 78 patients were taken 53 were HBsAg Positive (test) and 25 were HBsAg negative (control) all blood samples were subjected to estimation of ALT and quantification genotyping of HBV. The HBV genotypes were characterized through A-F. 43 specimens were subjected for HBV genotyping of which genotype D was most prevalent and was found in 23 patients (53.48%) followed by genotype A in O (23.33%) patients. Genotype B, C and E were found in 05 (11.62%), 02 (4.65%) patients respectively and a rare genotype F in 1 (2.33%) was also found correlation. There is no significant (p=0.121) between various HBV genotype and HBV DNA load. But significant correlation (p=0.012) was found between HBV genotype D and A and viral load. There was no association found between HBV genotype and serum ALT levels (p=0.575).