DOI: 10.36347/sjams.2018.v06i10.068

Pages: 4015-4022

Otitis media (OM) is an inflammatory disease of middle ear mucosa. Its significance lies in the dreaded complications and chronicity associated with the disease process. The present study was a cross sectional study conducted in the Department of Microbiology in association with the Department of ENT, AIMSR, Bathinda, over a period of one year. 131 clinically diagnosed cases of otitis media fulfilling both clinical and microbiological criteria, attending ENT department were studied. Two sterile swabs were used to collect ear discharge from each patient. First swab was used for direct microscopy by Gram staining and KOH mount. Second swab was used for culture onto MacConkey agar and Blood agar media. After incubation for 24 – 48 hours the plates were observed for growth, and isolates after confirmation were further subjected to antibiotic sensitivity testing. A total of six bacterial genera were isolated. Highest number of organism isolated was Pseudomonas aeruginosa, 45 (38.46%), followed by Staphylococcus aureus 39 (33.33%), Escherichia coli 14 (11.97%), Klebsiella pneumoniae 12 (10.26%), Coagulase negative Staphylococcus 4 (3.42%) and Enterococcus faecalis 3 (2.56%). Pseudomonas aeruginosa showed maximum sensitivity to Colistin, Imipenem and Polymyxin B (100%).Maximum resistance was observed to Levofloxacin (31.11%). Staphylococcus aureus showed maximum sensitivity to Teicoplanin and Vancomycin (100%). Maximum resistance was seen for Erythromycin (48.72%), followed by Cotrimoxazole (46.15%). Bacterial predominance and antibiotic susceptibility pattern changing over time, makes continuous and periodic surveillance necessary in guiding appropriate antibacterial therapy.