DOI: 10.36347/sjams.2023.v11i11.011

Pages: 1926-1933

Background and aim: Infertile couples are increasingly turning to Assisted Reproductive Technology (ART) for conception. Unfortunately, many of these women do not achieve clinical pregnancy and several factors such as hormonal imbalance, abnormal ovarian response, oxidative stress and senescence have been implicated. This study seeks to evaluate ovarian response to gonadotropin stimulation and the effect of oxidative stress and senescence on pregnancy outcome in women who enlisted in ART. Methods: A total of 46 women (mean age: 33.8±2.9 years and range 30-39) attending In-vitrofertilization (IVF) clinic at National Hospital Abuja were recruited for this study. Their blood specimens were collected four times at various stages during IVF treatment. Serum anti-mullerian hormone (AMH) was assayed using Cobas e411 auto-analyzer (ECLIA), total anti-oxidant status (TAS) was assayed using spectrophotometry method while Inhibin B was assayed using ELISA technique. Data was analyzed using Statistical Package for the Social Sciences software. Results: Twenty-two subjects 22/46(47.8%) were high responders, 11/46(23.9%) were normal responders, while 13/46(28.3%) were low responders to gonadotrophin stimulation after down regulation. The differences between the mean values of AMH across interval of sample collection days were statistically insignificant (p>0.05). The differences between the mean values of TAS and INHB across interval of sample collection days were statistically insignificant (p>0.05). There was a positive correlation (r=0.443, p < 0.002) between AMH and number of oocytes produced. A total of 16/46 (34.8%) of subjects were clinically pregnant while 30/46 (65.2%) achieved no pregnancy. Conclusion: Total Antioxidant Status and AMH levels appear to have affected pregnancy outcome. Therefore, TAS and AMH assays should be included as part of pre-IVF screening so as to improve pregnancy outcome in IVF treated women. Relevance for patients: This is a cross-sectional ....