DOI: 10.36347/sajb.2018.v06i05.007

Pages: 429-437

The aim of present study was to investigate in vitro antioxidant and anti‐ inflammatory potential of methanolic extract of flowers of Punica granatum Linn. (Family: Punicaceae). Antioxidant activity was studied using 1, 1‐Diphenyl‐2‐ Picrylhydrazyl radical scavenging activity (DPPH), superoxide radical scavenging activity (SRSA), total reducing power (TRP) assay, nitric oxide radical scavenging activity (NOSA), anti-lipid peroxidation (ALPO) activity, ABTS radical scavenging Activity and hydrogen peroxide scavenging activity (HPSA). Ascorbic acid was used as standard antioxidant. In•vitro anti‐inflammatory activity was evaluated using membrane stabilization assay and at different concentrations. Diclofenac was used as a standard drug for the study of anti‐ inflammatory activity. Linear regression analysis was used to calculate IC50 value. Results showed that, the extract exhibited significant total antioxidant, DPPH, SRSA, TRP, NOSA, ABTS and HPSA with IC50 value. Extract also showed in•vitro anti‐inflammatory activity by inhibiting the Red Blood Cells membrane stabilization with the IC50 values. This study revealed that the methanolic flower extracts of Punica granatum Linn. Has demonstrated significant antioxidant and anti inflammatory activity.