DOI: 10.36347/sajb.2018.v06i06.001

Pages: 446-451

Our aim is to produce biopolymers by using Bacillus cereus isolates. And objectives are soil Samples were collected from industrial effluents of pydibhimavaram region, Srikakulam. Screening for PHB producing bacteria was done by serial dilution of various soil samples. PHB efficiencies of these isolates were analyzed by Law and Slepecky method. Efficient PHB bacilli were studied by standard morphological and biochemical methods for identification of the isolate as Bacillus Cereus SKC, which was found to be producing more PHB. Bacillus cereus isolated from industrial effluents of Pydibhimavaram region, Srikakulam was used in this study. Stock cultures were maintained at 40 C by periodic transfer on nutrient agar slants. Serial dilution then followed bymorphological tests like gram staining, Spore Staining Capsule Staining and various biochemical tests were performed for screening of isolates. The optimization of the physiological parameters i.e. the pH, Temperature, Carbon, Nitrogen, Phosphorous sources were performed for the enhanced production of Poly beta hydroxyl Butyric acid (PHB). The determination of the amount of PHB was performed chemically. Screening for PHB producing bacteria was done by serial dilution of various soil samples, collected from industrial effluents of pydibhimavaram region, Srikakulam, which resulted in isolation of four isolates. PHB efficiencies of these isolates were analyzed by Law and Slepecky method that resulted in selection of Gram positive endospore bearing bacillus. Efficient PHB bacilli was studied by standard morphological and biochemical methods that finally identified the isolate as Bacillus Cereus SKC , which was found to be producing more PHB. Culture conditions for better PHB yields by Bacillus Cereus SKC was studied by optimizing pH, temperature, nitrogen source, carbon source, phosphorous source. The results of this study concluded that the isolate is efficiently producing PHB at a pH 7.5 and temperature 400C. Among car