DOI: 10.36347/sajb.2018.v06i06.005

Pages: 465-470

Antibiotic resistance among gram negative bacteraemia is a huge problem in health care settings. Detection of extended spectrum beta lactamases (ESBL), Amp C and carbapenemases would help implementing empirical treatment in bacteremia patients. There is a need of a simple and inexpensive screening test for multidrug resistant isolates. A total of 125 multidrug resistant isolates of Enterobacteriaceae were screened by twelve disc method and confirmed by the modified Hodge test and imipenem EDTA method. Among the 125 multidrug resistant isolates, Klebsiella pneumoniae was the most common 58( 46.4% ) followed by Escherichia coli 31(24.8%).The production of ESBL, MBL, KPC and Amp C were 61.6%,22.4%,7.2% and 32% respectively. The presents study indicates the burden of ESBL, Amp C and Carbapenemase production among Enterobacteriaceae using 12 disc method in blood culture isolates. The 12 disc method is a simple, cost effective and easy screening method for multidrug resistant isolates.