DOI: 10.36347/sjams.2020.v08i05.034

Pages: 1339-1343

This work was aimed at testing the effectiveness of a locally sourced and fortified lipid solvent, on Influenza A Disease virus in some infected poultry birds at the National Veterinary Research Institute, Vom (NVRI) Plateau State of Nigeria. The research was designed by randomly blocking (during lipid dilution process) thirty five eggs (35) of 9 (nine) day old Hen embryonated eggs into groups of seven (7) based on lipid dilutions viz; six (6) lipid dilution groups and the control group. Dilutions from 10-5 to 10-10 were used for the assay. 0.1ml of the respective dilutions was transferred to sterile Bijou bottles labelled with the corresponding lipid solvent dilution. Thereafter, equal volumes of 4HA unit concentration of neat suspension of influenza virus strain (A/PR/6/32) was added to each bottle. Five eggs were labelled for each lipid solvent dilution. 0.1ml lipid/virus mixtures were randomly inoculated into five (5) eggs bearing the respective lipid dilutions. Five eggs were inoculated with 0.1ml each of the virus suspension to serve as virus control. Eggs were incubated at 370C for 72 hours. Each egg was cut open after incubation and allantoic fluid tested for Heamagglutination reaction using 10% chicken RBC. The results revealed that the locally sourced lipid solvent has neutralizing effects on influenza virus by the Heamagglutination inhibition technique (HAI). Therefore Neutralization titre = 10-5.6 influ.50/0.1mL. This study findings suggest that the locally sourced lipid solvent is naturally microcidal (killing harmful bacteria, yeast, fungus, and viruses).