DOI: 10.36347/sajb.2018.v06i08.003

Pages: 579-584

The local cultivar of Singgalang cabbage is an endemic vegetable in West Sumatra Province, Indonesia, it has three variants, namely Biaso, Batang Hitam and Senggan which is favored by consumers. This local cultivator is threatened with extinction due to lack of interest by farmers regarding the low productivity and susceptibility to pest and disease attacks compared to modern cultivars. To conserve the local germplasm and its use as a breeding material have been analyzed genetic variation and genetic differentiation by RAPD technique using 17 primers. The result showed that three primers (OPAC-11, OPA-02, and OPA-9) were able to amplify DNA and produce polymorphic bands. The percentage of polymorphic bands using OPAC-11, OPA-02 and OPA-09 was 100%, 90%, and 100%, respectively. The Batang Hitam variant has highest heterozygosity value (H) is 0.2725 with 0.3932 of the Shanon diversity index value (I), while the Biaso variant shows the lowest value of H (0.1400) and I (0.2070). Genetic variation between variants (Gst) is categorized as low (0.3917), and the gene flow (Nm) is high (0.7763). The cluster analysis showed that between Biaso and Senggan variants have the most distant genetic distance of 0.4057, and followed by Biaso and Batang Hitam variants with the genetic distance of 0.3273.