DOI: 10.36347/sajb.2016.v04i05.010

Pages: 413-421

Colletotrichum acutatum is one of the pathogenic organisms of anthracnose disease of chili fruit, which was isolated from the chili species Capsicum annuum. The pathogen was identified from the observation of its colony size, shape, colour, mycelium, conidiophore, conidia, hyaline, spore, acervuli, appressoria, and setae in the PDA culture. The pathogenic organism produced antibacterial activity when cultured in PD liquid broth medium. The highest production of antibiotic from the organism Colletotrichum acutatum was found at pH 6.0 and 270C. The crude chloroform extracts showed antibacterial activity against a number of Gram positive and Gram negative bacteria. TLC analysis showed the presence of three fractions of crude chloroform extracts from C. acutatum. The minimum inhibitory concentration (MIC) of the crude chloroform extract from C. acutatum against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Shigella sonnie were 256 µgm/ml, 128 µgm/ml, 128 µgm/ml and 64 µgm/ml respectively. The LD50 (Lethal Dose) values of the cytotoxicity assay over brine shrimp of the crude chloroform extract from C. acutatum was found 956.02 μgm/ml.