DOI: 10.36347/sajb.2019.v07i02.007

Pages: 76-81

Long non-coding RNA (lncRNA) is a kind of RNA segment longer than 200 nucleotides (nt), with little or no protein-coding capacity. In our previous research on the male sterility of cattleyak, a differentially expressed (DE) lncRNA (id: NONBTAT010258) named lncRNANONBT-258 was identified from the testicular tissues of yak and cattleyak through transcriptome sequencing. Genome alignment revealed that lncRNANONBT-258 was located between RND2 and BRCA1 genes on bovine chromosome 19 and encoded by one exon. RT-PCR confirmed the expression of lncRNANONBT-258 and its target gene BRCA1 in the testis of yak and cattleyak, and RT-qPCR showed that the expression trend of these two molecules was consistent with the sequencing results. Furthermore, molecular cloning and resequencing indicated that the lncRNANONBT-258 presented higher similarity among different cattleyak and yak individuals