DOI: 10.36347/sajb.2019.v07i04.002

Pages: 166-169

This study aimed to detection Toxoplasma gondii in eggs of different sources, using Real- Time Polymerase Chain Reaction (PCR). A total of 300 chicken’s eggs samples were collected from different source (native chickens 100, Iraqi eggs production fields100, trade-imported eggs 100). Genomic DNA extracted from inner components of eggs (chalaza) and tested by Real Time PCR using specific primers and TaqMan probe targeting B1 gene of parasite. The native eggs samples showed 64 of 100 (64%), 8 of 100 (8%) of Iraqi eggs production fields and 4 of 100 (4%) of imported eggs were detected as positive, the others samples was negative. Statistical analysis showed highly significant differences between the different sources when using Real - time (PCR) technique to detection Toxoplasma gondii under p ≤ 0.05.These results is indicate that Toxoplasma gondii infection in native eggs is relatively high in compare with other eggs sources.