DOI: 10.36347/sajb.2019.v07i08.003

Pages: 330-333

Probiotics are live microorganisms which impart into gastro-intestinal tract and are beneficial to the health of consumer. Some probiotics have potential to boost the immunity as well as inhibit the growth of bacteria and fungi. This study was designed to isolate and identify potential Lactobacillus isolates from dairy product and also evaluate the fungistatic activity of these bacteria against environmental isolates which are involved in food poisoning; Candida specie and Aspergillus niger. Food spoilage by fungi and human fungal infection are now considered as a major problem and cause great economic losses. The chemical which are used in food preservation are very expensive and unaffordable, so, to get rid of these problems we are now moving to bio preservation and there is a high demand of consumers for natural preserved food products which protects the food and human health as well. In this study, lactobacilli were isolated from different dairy product (yogurt and raw milk) which is the cheapest source to isolate fungi inhibitors. The fungistatic activities of these isolates were evaluated by Agar well-diffusion method. Zone of inhibition were measured in millimeter (mm), the highest inhibition zone was observed for Aspergillus niger that is 24.5mm, while 25.5mm was the highest for Candida specie. This indicates that the lactobacillus possess fungistatic property and contain antifungal compound which can inhibit the growth of fungi. The isolates of lactobacillus were more effective against Candida spp. The result of present study revealed that fungal spores and fungal growth was lessen by the cell-free neutralized filtrates (CFNF) of Lactic acid bacteria, so by using these antifungal agents we can preserve the food product for long time economically and can boost the immunity and health of an individual as well.