DOI: 10.36347/sjavs.2016.v03i05.004

Pages: 358-369

A simple and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with UV detection for determination of pesticide residues (Valifenalate, Kresoxim-methyl, Ethoxysulfuron, Topramezone and Tolfenpyrad) in different crop commodities. The evaluated parameters include the extracts by phenyl, silica gel and alumina solid phase extraction cartridges using methanol, ethyl acetate, distilled water and tetrahydrofuran solvents. The method was validated using croup samples spiked with Valifenalate, Kresoxim-methyl, Ethoxysulfuron, Topramezone and Tolfenpyrad at different fortification levels (0.01 and 0.1 μg/g, 0.05 μg/g and 0.5 μg/g, 0.03 μg/g 0.3 μg/g 0.01 and 0.1 μg/g and 0.03 μg/g 0.3 μg/g). Average recoveries (using each concentration six replicates) ranged 84-95%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01- 10.0 μg/mL and limit of detection (LOD) and limit of quantification (LOQ) range were 0.003 to 0.02μg/g and 0.01 to 0.05μg/g respectively. Subsequently the crop residue samples have been analyzed through HPLC.