DOI: 10.36347/sjavs.2016.v03i05.005

Pages: 370-377

A field experiment was conducted at the experimental field of Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur during October 2010 to April 2011 to detect different viruses in 26 pumpkin breeding lines. The test lines were evaluated under natural condition for virus reaction. Different types include in pumpkin leaves of the test lines, of which the most prevalent symptoms were fern leaf, mosaic, leaf distortion, chlorosis, and vein banding and chlorotic spot. Four different viruses were detected in those of symptomatic pumpkin leaves by Double antibody Sandwich ELISA (DAS-ELISA) techniques. Detected viruses included Papaya ringspot virus-watermelon strain (PRSV-W), Watermelon mosaic virus 2 (WMV2), Cucumber mosaic virus (CMV) and Zucchini yellow mosaic virus (ZYMV). These viruses caused fern leaf, mosaic, chlorosis and vein banding and leaf distortion symptoms, respectively. ELISA results showed PRSV-W and ZYMV were the most prevalent virus followed by CMV and WMV2 related to number of infected lines. Among the lines, seven (Pk13-1-1, Pk20-2-1, Pk02-2-1, Pk19-4-1, Pk54-4-12, Pk01-10-9-4 and Pk106) did not react to any of the four antisera tested. Of the rest line, five (Pk55-2-2, Pk05-1-2, BARI mistikumra 1, BARI mistikumra 2 and Pk101) were positive to PRSV-W; five (Pk05-4-1, Pk05-8-2, Pk75-1, Pk07-4-7 and Pk102) ZYMV, two (Pk34-4-3 and Pk67-1-9) CMV, and only one (Pk105) WMV2. Six lines (Pk31-2-4, Pk37-1-4, Pk61-1-1, Pk04-7-12-3, Pk05-7-11-8 and Pk107) showed positive reaction to Potyvirus group while negative to four antisera tested. Fourteen indicator plants were mechanically inoculated with four detected viruses (PRSV-W, WMV2, CMV and ZYMV). Results of the host range test suggested that PRSV-W, WMV2, CMV and ZYMV were sap transmissible to plant species belonging to Cucurbitaceae, Chenopodiaceae, Solanaceae, Leguminosae and Amaranthaceae family. Cucurbitaceous and Solanaceous host plants were also the systemic host for all virus