DOI: 10.36347/sjavs.2016.v03i06.005

Pages: 424-428

This study was carried out to investigate the seroprevalence of brucellosis in dairy cattle in Port Sudan, the Red Sea state, the Sudan. Two hundred and fifteen blood samples were collected from three locations in Port Sudan locality taking the density of animals in each location in account. Sera samples were tested by the following tests: rose Bengal plate test (RBPT), modified rose Bengal plate test (mRBPT), serum agglutination test (SAT) and competitive enzyme-linked immuosorbent assay (cELISA). The seroprevalences were 13.0% by RBPT and 21.0% by mRBPT. Furthermore, SAT confirmed the positivity of 93% of the RBPT- and mRBPT-positive samples with titers ranging from 20 IU/ml to 1488 IU/ml. The cELISA confirmed the positivity of 27, 21, and 25 of the RBPT, mRBPT, and SAT positives, respectively. The results suggest that the mRBPT is more sensitive than the RBPT in identifying brucellosis-positive cattle. Therefore, it is recommended that mRBPT to be used in the routine diagnosis of brucellosis in dairy cattle.