DOI: 10.36347/sjavs.2017.v04i01.001

Pages: 1-5

Study was conducted at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University Tandojam to detect estrus in time in Kamohri goat. In goats measuring plasma P4 concentration is most important to monitor luteal functions. In present study the forty adult Kamohri goats were selected and utilized in study. The goats were divided at random in to four equal treatment groups. Goats of group A received progesterone on day 0 and PGF2α along with GnRH both on day 10th, goats in group B were given PGF2α on D-0 and repeat dose of PGF2α along with GnRH on D-10th. While animals of group C were offered 50 gm of dry date fruit with 50 gm of Ajwain both daily for 10 days, whereas goats of group D received no any treatment and served as control. The goat does were treated accordingly and closely observed for estrus. The blood samples were collected from each goat to determine the estrus on the basis of plasma progesterone concentration in Kamohri goat. The serum progesterone concentration was measured by Enzyme Linkage Immuno Sorbent Assay (ELISA) kit. The results revealed that the goats of treatment groups A, B and C induced estrus 100%, irrespective of estrus synchronization treatment. However none of the goat in group D shown estrus during trial time period. The mean progesterone concentration level at day of estrus was recorded as 0.46±0.07, 0.34±0.06 and 0.36±0.05 ng/ml in treatment group A, B and C respectively and 1.38±0.04 ng/ml in control group of animal in Kamohri goat does. There were no any significant difference (P ≥0.05) was found between the treated and control group. It was concluded that the, P4 concentration provides rapid and simple method and it could be applied to monitor estrous cycles and P4 analysis can be applied as a useful tool to help to herds’ men and veterinarians in the determination of estrus so that more efficient breeding program could be developed and implemented in the flock at farm leve