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Scholars Academic Journal of Biosciences | Volume-12 | Issue-04
Effects of Saline-alkali Stress on the Growth of Transgenic Sorghum Overexpressed SbFLA15 in Bud Stage
Li Luo, Lingxin Kong, Wenhua Lv, Shulei Wang, Shanwen Yao, Ruoyu Zhang, Xinyu Zhuang, Yuao Zhou, Yuke Zhang, Xiao Wu, Ning Cao, Lingyan Dai
Published: May 13, 2024 | 85 95
DOI: 10.36347/sajb.2024.v12i04.003
Pages: 76-83
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Abstract
In this study, wild-type sorghum P898012 (WT) was used as a control to investigate the effect of salinity stress on the growth of the overexpressed SbFLA15 sorghum lines (OE) in bud stage. The results of the concentration screening of saline-alkali stress solution showed that 75 mM (NaHCO3 : Na2CO3 is 5:1, pH 9.63) was more suitable to simulate stress. Under saline-alkali stress, the germination and growth of different transgenic strains showed different resistance, with OE 1 being the strongest and OE 7 the worst. OE 1 was selected as the research subject to further investigate the impact of saline-alkali stress on the growth of overexpressed SbFLA15 sorghum lines during the bud stage, and the results showed that the relative germination potential, bud length, germination index, fresh weight and dry weight of OE 1 were better than those of WT, showing stronger ability to resist saline-alkali stress.