DOI: 10.36347/sjams.2020.v08i09.028

Pages: 2120-2126

Pyocyanin is a biologically active phenazine produced by Pseudomonas aeruginosa. Pyocyanin is a blue redox-active secondary metabolite and a member of the large family of the tricyclic compounds. “Phenazines” Because of its solubility in chloroform it can be easily isolated. Pyocyanin or 1-hydroxy-5-methylphenazine is considered as a resonance hybrid of the mesomeric forms of N-methyl-1-hydroxyphenazine is capable of undergoing a two-electron reduction to a colourless product, leukopyocyanin. It is believed to bestow P. aeruginosa with a competitive growth benefit in colonized tissue and is also thought to be a virulence factor in diseases such as cystic fibrosis and AIDS where patients are commonly infected by pathogenic Pseudomonads due to their immunocompromised state. Pyocyanin is also a chemically stimulating compound due to its unusual oxidation-reduction activity. Phenazine-1-carboxylic acid, the predecessor to the bioactive phenazines, is produced from chorismic acid by enzymes determined in a seven-gene cistron in Pseudomonas aeruginosa. Phenzine-1-carboxylic acid is whispered to be converted to pyocyanin by the sequential actions of the putative. S-adenosylmethionine dependent N-methyltransferase PhzM and the putative flavin-dependent hydroxylase PhzS. Here we report the Predicted 3D Structure of PHZ S.