DOI: https://doi.org/10.36347/sjams.2024.v12i08.002

Pages: 920-924

Objectives: The aberrant expression of related-fibrosis factors were proved in detrusor of female rats model with bladder outlet obstruction (BOO), but the relationship between hypoxia inducible factors and male gender and related regulation pathways over detrusor fibrosis has were uncommonly explored resulting to the purpose of this study. Methods: A total of 12 Sprague-Dawley (SD) male rats weighing 200 to 250 g were used to induce the partial BOOO (PBOO) model of male rats. After 3 weeks of PBOO construction in male rats, those rats bladder of PBOO and Sham groups were excised and weighed. The mRNA expression of Piezo1, HIF-1α, connexin43, and TGF-β1 in detrusor were measured by Quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: After 3 week of PBOO construction in male rats, significant heavier bladder weight was found in PBOO rats compared to that of sham operation (710.7mg±45.5 vs 218.1mg±26.7, P＜0.001) and the mRNA expression of Piezo1, HIF-1α, connexin43, and TGF-β1 was significantly increased in detrusor of PBOO rats compared to that of sham operation. Conclusions: It was suggested that those fibrosis markers in detrusor might be not impacted by different background of sexual hormones between male and female rats. More research should focus on the influence of interaction between ncRNA and fibrosis factors, particularly HIF1A-AS1/HIF-1α pathway and TGF-β1/Smads pathway, on detrusor stability and contractility due to BOO in patients with BPH.