DOI: 10.36347/sjavs.2018.v05i02.003

Pages: 65-72

Mastitis and pathogen related differences also depend upon the ability of the host’s immune defense system. MHC play important role in providing defence against pathogens. Defensin plays a very important role in defending against the various microorganism. Hence, the genes encoding those regions seems to be possible genetic markers for resistant or susceptibility to various microorganisms. In the present study BoLA-DRB3 gene were studied. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used for identification of polymorphism in both the genes. Using Hae III restriction enzyme 6 different types of pattern has been observed in cattle, whereas in buffalo 7 types of pattern was observed in healthy and mastitis samples. Using RsaI restriction enzyme 10 different types of pattern has been observed in case of cattle, whereas in buffalo 8 types of pattern was observed in healthy and mastitis samples. Significant differences have been found between cattle and buffalo breed studied. RsaI –I and RsaI-f was found in healthy samples, but was absent in mastitis samples. Collectively, the finding in the present study states the use of alleles HaeIII-c, RsaI-l and RsaI-f as a reference for more detailed mechanistic studies.