DOI: https://doi.org/10.36347/sajb.2024.v12i09.003

Pages: 294-300

This study explores the antioxidant and antibacterial activity of Centella asiatica herb extract with the aim of identifying the optimal extraction method to maximize its biological potential. Given the increasing bacterial resistance to antibiotics and the need for safer natural alternatives, this research focuses on using ethanol as a solvent at various concentrations for the extraction of bioactive compounds. The research methodology involves extraction with 25%, 50%, and 75% ethanol, followed by the evaluation of antioxidant and antibacterial activity against Escherichia coli. The results indicate that ethanol concentration in Centella asiatica extract significantly affects its antioxidant and antibacterial potential. The 75% ethanol extract exhibited the highest antioxidant and antibacterial activity, with significant differences observed in the ANOVA test (p-value 0.000). The study concludes that the 75% ethanol extract of Centella asiatica possesses high therapeutic potential, with higher extract concentrations yielding stronger antioxidant and antibacterial effects.