DOI: https://doi.org/10.36347/gamj.2024.v05i04.003

Pages: 119-125

Diabetic nephropathy (DN) is the leading cause of end-stage renal disease worldwide. The present study was performed for the Molecular detection of insulin-like growth factor 1 single nucleotide polymorphisms (rs10860860) of patients with type 1 and type 2 diabetes mellitus and diabetic nephropathy in Al-Qadisiyah Province. To achieve this aim, 100 samples were collected, divided into 2 groups: those who are 40 Type 1 diabetes patients (20 T1DM with nephropathy and 20 T1DM without nephropathy); 40 Type II diabetes patients (20 T2DM with nephropathy and 20 T2DM without nephropathy); and healthy controls. The DNA was extracted from whole blood for all specimens. Genotyping was carried out by the polymerase chain reaction-sequencing technique. The digested products were analyzed by agarose gel electrophoresis. The genotypes of insulin-like growth factor 1 showed a significant association (P < 0.05) with type 1 and type 2 diabetes mellitus and diabetic nephropathy. Genotypes and allele frequencies related among T1DM with nephropathy and healthy controls, risk analysis revealed that homozygous TT genotype was a significant risk factor (OR=9.75), and heterozygous A/T genotype was also a risk factor significant with an odds ratio of 6.5, which means that patients with a heterozygous TT genotype are approximately ten times more likely to develop the disease than those with other genotypes. Genotype and allele frequencies between T1DM without nephropathy and healthy controls, risk analysis revealed that homozygous TT genotype was a significant risk factor (OR=6.5), and heterozygous A/T genotype was also a significant risk factor with an odds ratio of 7.8, meaning that patients with a heterozygous TT genotype are approximately seven times more likely to develop the disease than those with other genotypes. Whereas genotypes and allele frequencies related to the IGF-1 (rs10860860) SNP among T2DM with nephropathy and healthy controls, risk analysis revealed that homozygous .....