DOI: 10.36347/sjavs.2018.v05i04.011

Pages: 255-265

Abstract: Illustration of distinct cause of Fasciolosis in different geographic regions has been the main wide reaching concern from veterinary medicine and public health perspectives. Present exploration was done for accurate diagnosis and evolutionary analysis of Fasciola species based on internal transcribes spacer second (ITS2), 5.8s rDNA. A total of 348 samples were used in this study, including each 87 fecal and bile samples from Sahiwal cattle and Nili Ravi buffaloes. ITS2, 5.8s rDNA gene region was amplified in 300bp through PCR from Fasciola hepatica eggs recovered from fecal and bile samples. Phylogenenetic analysis was concluded using maximum likelihood method and 24 different models were used. MEGA 6.0 was used to create multiple alignment of ITS1 and ITS2 gene for all Fasciola isolates, which allowed to create multiple alignments by ClustalW, using discrete Gamma distribution (+G), and evolutionary invariable (+I) and percent identity between the isolates was determined. The haplotypes structure and sequence variation indices were intended using DnaSP version 5.10.01, including nucleotide diversity, number of polymorphic sites, number of haplotypes and haplotypes diversity. Hence these investigations provide comprehensive approaches to formulate systemic searches for rising focus of human fasciolosis in Pakistan.