DOI: 10.36347/sjavs.2018.v05i10.002

Pages: 541-547

Abstract: Genetic diversity plays important role for any successful genetic improvement programs. This investigation used to assess the genetic variability of twenty Sudanese sweet genotypes which collected from different regions among country. In this study fourteen SSRs markers used, the result indicated13 (93%) of SSRs primers were polymorphic, generated 29 alleles 26(90%) polymorphic. PIC value ranged between 0 to 1 with an average 0.45, Genetic dissimilarity between genotypes ranged from 0.13 to 1.14 with mean 0.78. based on similarity, Dendrogram was constructed and three clusters were grouped, the 20 sweet sorghum genotypes was revealed several pattern of variation, although some of them share same geographic location. The current study indicates that, using of molecular markers, especially SSRs can be used in sweet sorghum improvement to accelerate the breeding program. Both the genetic similarity and heterozygosis information among all genotypes revealed by this study it will be helpful in sweet sorghum breeding programs to improve yield , ethanol and sugar production and maintain broad genetic diversity. These results suggest a need for further evaluation at the level of molecular genetic diversity through the application of high throughput genome sequencing and genotyping by sequencing to improve sweet sorghum, as well as effective use of these tools in molecular breeding programs.