DOI: https://doi.org/10.36347/sajb.2025.v13i01.003

Pages: 21-25

Vibrio cholerae N-acetylglucosamine binding protein (GbpA), a secretory colonization factor which is essential for adherence to chitin rich surfaces in environment as well as in intestinal lining. It was previously shown to interact with Toll like receptor 2 on host cells and induce inflammatory response. However, the detailed image of the GbpA-TLR2 interaction is yet to elucidate. In this study using confocal imaging we have found that GbpA co-localize with TLR2 on host cell surface. The predicted model of GbpA-TLR2 complex using molecular docking analysis revealed that the concave surface of TLR2 was involved in H-bond interaction with domain 1 of GbpA. With this study we provide insight into V.cholerae specific host-microbe interaction involving GbpA and TLR2.