DOI: 10.36347/sjams.2021.v09i12.029

Pages: 1939-1943

Background: Cholelithiasis is the most common disease of the gall bladder afflicts 10% to 20% of adult populations in developed countries. It may develop into carcinoma by the dysplasia carcinoma sequence. Over 80% of invasive gallbladder cancers are present in areas adjacent to the carcinoma in situ and epithelial dysplasia. Ki67 expression is correlated with the aggression of various histopathological changes in the epithelium of the gallbladder and may act as prognostic marker in gallbladder carcinoma. CD34 expression is also increased in severe dysplasia and carcinoma. This study aims to assess the proliferative activity and microvascular density associated with lesions like hyperplasia, metaplasia, dysplasia and malignant lesions in cholecystectomy specimens. Materials and methods: It is a Hospital based cross sectional study conducted in department of Pathology, Regional Institute of Medical Sciences, Imphal, Manipur for two years from October 2017. Cholecystectomy specimens with features of metaplasia, dysplasia and malignancy are analysed. Immunostaining of the sections done with Ki 67 and CD34 and interpreted both qualitatively and quantitatively. Statistical Analysis done using SPSS IBM VERS.21. Results and observations: A total of 150 cholecystectomy specimens are studied with Male to female ratio is 1:9. Gallbladder carcinoma seen more commonly in females. The most common type is adenocarcinoma NOS with mean age of 41.9 years +/-14. Conclusion: Determination of immunohistochemical expression of Ki67 and CD34 may act as prognostic marker and help in assessing the aggressiveness of premalignant and malignant lesions of gall bladder.