DOI: https://doi.org/10.36347/sajp.2025.v14i02.001

Pages: 9-13

Upon completion of work performed on the Endosafe® Multi-Cartridge System (MCS), it was discovered that some amphiphilic, micelle-forming substances such as phosphatidylcholine (PC) may inhibit Limulus Amebocyte Lysate (LAL) chromogenic assay. It was hypothesized that the presence of micelles in the solution is responsible for the inhibition. This assumption was tested on Triton X-100 solutions, and it was shown that the Triton X-100 at concentrations less than its Critical Micelle Concentration (CMC) did not inhibit the assay, but at the concentrations above the CMC it did. Minimum Detergent Dilution (mDD) is the threshold quantity that tells us how many times the sample must be diluted to avoid inhibition. We concluded that the Maximum Valid Dilution (MVD) should be greater than mDD to prevent inhibition of recovery. If the inequality MVD > mDD is not true, the simple dilution will unlikely eliminate inhibition.