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Scholars Academic Journal of Pharmacy | Volume-14 | Issue-05
Evaluation of Anti-Inflammatory Activity of Gloriosa Superba Using Various In-Vitro Methods
Manikanta K, Ramya Sri M, Bhavana B, Jhansi C, Divya Sai Sri B, Manohar Babu Sitty, Sai Kumar Makka, S. Bhagya Nagalakshmi, Sai Kumar Makka, Manohar Babu Sitty
Published: June 2, 2025 | 141 105
Pages: 91-96
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Abstract
Background: Inflammation is a biological response to harmful stimuli, and traditional medicinal plants have been widely explored for their anti-inflammatory potential. Gloriosa Superba, a medicinal plant, is known for its therapeutic properties. This study evaluates the in-vitro anti-inflammatory activity of Gloriosa Superba tubers. Methods: The anti-inflammatory activity of the ethanolic extract of Gloriosa Superba tubers was assessed using the human red blood cell (HRBC) membrane stabilization method, including heat-induced hemolysis and hypotonicity-induced hemolysis, along with an egg albumin denaturation assay. Acetylsalicylic acid (ASA) was used as a standard reference drug. Results: The crude ethanolic extract exhibited significant anti-inflammatory activity in a concentration-dependent manner. The heat-induced membrane stabilization method revealed that the extract (500 μg/ml) and ASA (500 μg/ml) exhibited 52.667% and 78% inhibition, respectively. The hypotonicity-induced hemolysis method showed 35.67% and 59% inhibition of RBC hemolysis, respectively. The egg albumin denaturation assay demonstrated that the crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) inhibited egg albumin denaturation by 60% and 97.12%, respectively. Conclusion: The tubers of Gloriosa Superba exhibit promising anti-inflammatory activity, supporting their potential medicinal application. Further in-vivo studies are recommended to establish its efficacy and safety.