DOI: https://doi.org/10.36347/sajb.2026.v14i03.003

Pages: 201-217

The mosquito-borne flavivirus known as the Zika virus is now a growing worldwide public health problem. An RNA virus called (ZIKV) has spread through mosquito bites. There are currently no Zika vaccinations on the market, and the handful that are are still in scientific studies. Since little is currently recognized about the toxicity, genetic variety, and effects of ZIKV contamination, there's an urgent want for a preventive vaccination against the virus. The traditional vaccination strategy administers live-attenuated or inactivated vaccinations, which are dangerous and cause the illness to worsen. An immunoinformatics method changed into used to design and create a multi-epitope vaccine against Zika, taking into account the necessity for a more secure vaccination. In order to anticipate MHC class-I and class-II epitopes, the ZIKA protein serine protease ns3 was taken from the database and employed in various analyses which include physiochemical analysis, allergenicity, antigenicity, and toxicity. A 50S ribosomal protein L7/L12 became inserted on the N-terminal of the vaccine, followed by CTL and HTL related by corresponding linkers. For the developed vaccine, linear B-cell epitopes were predicted after which physiological parameters had been evaluated. The development was non- allergic and antigenic. It became located that the vaccination becomes safe for allergic reactions and produced an antigen response. After that, the vaccine design became modeled and docked on the TLR4 receptor to see if it would cause an immunological response. For fifty ns, the docked complex turned into further simulated. Ultimately, the vaccine design was cloned in-silico into the pet28a (+) vector in order to use His-tag for affinity purification. It's likely that the vaccine will be advanced as a Zika vaccine. To make certain the legitimacy of its expression efficiency, the construct underwent in silico cloning. The creation of vaccines may continue greater speedy thanks to those comp