DOI: https://doi.org/10.36347/sajb.2026.v14i09.005

Pages: 624-631

The primary aim was to evaluate the effect of dietary inulin and lactic acid bacteria (LAB) on the gut microbiota and immune response of Nile Tilapia (Oreochromis niloticus). Experimental group was given inulin and LAB at 2% of body weight. While control group was fed with commercial fish feed. Fish were treated with these three feeds for 2months. Culturing of bacteria and immunological assays were done to evaluate the effect of these feeds on O. niloticus. Physico-chemical parameters of the aquarium were maintained end-to-end during the trial period such as DO at 5-7mg/L, temperature at 24-28֯C and pH at 7.0-8.2. The quantitative outcomes from groups were compared using one way ANOVA. In all three-culturing media such as NA, TSA and EMB, the results indicated a significant increase (P<0.05) in bacterial colonies in the experimental group T1 when compared to the T2 and control group T0. Compared to the control group, the experimental groups T1 and T2. The T2 group had a significantly higher WBCs count, higher Hemoglobin value, neutrophils and lymphocytes are significantly higher count (P<0.05) than the control group. However, the immune system of fish was improved by using the probiotic in the fish diet and it also increases the body’s natural resistance to infection and facilitate in prevention of various diseases.