DOI: 10.36347/sjet.2020.v08i03.002

Pages: 42-47

Bovine parainfluenza virus type 3 (BPIV3) causes epidemic diseases in worldwide. A SYBR Green real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay was developed for the rapid detection and quantitation of BPIV3 in this study. Methods: A pair of specific primers was designed in the HN gene of this virus. Results: When comparing this assay with conventional RT-PCR, the rRT-PCR assay was 10 times more sensitive and could detect levels as low as 5.13×101 DNA copies of the BPIV3 DQ strain. All the standard shows a sharp and narrow dissolution peak at 78.8℃. The specificity of this technique was evaluated in three other bovine pathogens. Conclusion: This high sensitivity, specificity and simplicity of the SYBR Green rRT-PCR approach can be a more effective method than the conventional one for BPIV3 diagnosis and surveillance.