DOI: 10.36347/sajb.2020.v08i08.004

Pages: 249-254

Optimization of PCR is very important as it is a time taking hectic procedure which once done can save a lot of important time and confusion in genetic studies involving gene amplification using PCR. The CTLA-4 gene has been found to be associated with many autoimmune diseases including rheumatoid arthritis [1]. The present study was performed to optimize the Polymerase chain reaction (PCR) amplification of CTLA-4 gene from genomic DNA isolated from rheumatoid arthritis patients. The conditions were optimized for different parameters such as Taq DNA polymerase concentration, dNTPs concentrations, Primer concentrations, MgCl2 concentration and different enhancing agents. The results obtained were used to develop the protocol for amplification reaction. The study can be useful for researchers looking for quick amplification of the gene under study using the optimized conditions without going through the complex procedures.