DOI: 10.36347/sjams.2021.v09i01.008

Pages: 42-46

In India incidence of TB is 27, 90,000, mortality is 4, 23,000 and incidence of MDRTB/RR 1, 47,000. Early detection of TB is the key to successful treatment and reduction of disease transmission. The aim of this study is to determine the Rifampicin resistant, Mycobacterium tuberculosis by Gene Xpert in patient attending TB chest hospital, Hyderabad. All the Samples, Pulmonary and Extra pulmonary, received from clinically suspected cases of Tuberculosis, IP and OP during the study period between March 2018 to October 2018 were processed in the department of Microbiology using Xpert MTB/RIF test version 4.8 (Cepheid) as per the manufacturer’s instruction. Result: Prevalence of total MTB culture positive was 34.2 % (754/2202) and Rifampicin resistant was 13.7 % (103/754) in the total samples processed. Conclusion: Early detection of TB is the key to successful treatment and reduction of disease transmission. Gene Xpert was found to be useful assay for rapid detection of MTB within 2 hours with additional advantage of identifying Rifampicin resistance with high sensitivity and specificity.