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Scholars Journal of Agriculture and Veterinary Sciences | Volume-8 | Issue-07
Cloning and Knockout of YbhS belong to ABC Superfamily of Escherichia coli
Liu qing-peng, Sun chen-yu, Feng zhen, Wang Sheng-qi, Feng Zhen-Yue
Published: Aug. 3, 2021 |
125
92
DOI: 10.36347/sjavs.2021.v08i07.001
Pages: 72-75
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Abstract
ABC superfamily of bacteria is involved in the uptake of nutrients, excretion of metabolites, formation of bacterial biofilm, and is related to drug resistance. E. coli contains about 70 ABC transporters, equivalent to 5% of the genome. E. coli is predicted to have 11 export pumps, some of which are related to drug resistance. YbhFSR is one of them. In this paper, the permeable enzyme YbhS of the YbhFSR pump was systematically bioinformatics analyzed and gene knockout was carried out by Red recombination method, laying a foundation for subsequent studies on the function of YbhFSR transporter.