DOI: 10.36347/sjavs.2021.v08i10.001

Pages: 102-104

The alfalfa esterase was purified by ammonium sulfate precipitation method, cellulose method and sephadex method respectively. The result showed that the esterase activity purified by sephadex was higher than that of the other two purification methods. Infrared spectroscopy technology was used to study alfalfa esterase purified by different methods, it was found that absorption peaks of the infrared spectrum of crude extracts before purification was plentiful and disordered, and the absorption peaks of purified samples showed obvious and clear, indicating that all three methods could be used to purify esterase, it’s just the peak of esterase purity of sephadex was the highest.