DOI: 10.36347/sajb.2021.v09i11.008

Pages: 379-381

Salmonella typhimurium is a food-borne pathogen that causes gastroenteritis. Until now, antibiotics have been used to treat gastroenteritis. Nowadays, some antibiotics have become resistant to a variety of pathogens. Therefore, alternative treatments based on natural ingredients need to be explored. Lactic acid bacteria, or so-called probiotics, that are naturally found in human digestion have been studied for their antibacterial properties, such as bacteriocins. The purpose of this study is to see if bacteriocin from Bifidobacterium longum FNCC 0210 has any inhibitory activity against Salmonella typhimurium ATCC 14028 in vitro. Bacteriocin was extracted from B. longum as cell-free culture supernatant (CFCS) in MRS broth, which was neutralized at pH 6.5 with 1 N NaOH and heated at 100°C for 10 minutes. The antibacterial assay of B. longum bacteriocin against S. typhimurium was performed with the agar-well diffusion method with concentrations of 100, 50, 25, 12.5, 6.25% (v/v), and determined based on the diameter of the zone of inhibition (mm). The results of the tests revealed that B. longum CFCS 100% (v/v) had the highest antibacterial activity against S. typhimurium with intermediate category. This study provides information on the ability of B. longum bacteriocin that has antibacterial activity against food-borne pathogens.