DOI: 10.36347/sasjm.2016.v02i03.006

Pages: Page: 74-78

To determine whether blocking the mitochondrial permeability transition pore (MPTP) with cyclosporine-A (CsA) can restore cardioprotection in immature rabbit heart. On the Langendorff perfusion apparatus, isolated perfused immature rabbit hearts underwent 30 minutes of normothermic ischemia, followed by 120 minutes of reperfusion. 28 isolated immature rabbit hearts were randomly divided into four groups: control group(A), infused with Krebs-Henseleit solution (KHS); ischemic reperfusion group(B), CsA group(C), receiving the reperfusion of KHS +CsA; and Atr group(D),given the reperfusion of KHS+CsA+Atr. The postischemia myocardial function were assessed by the percentage recovery of heart rate(HR),left ventricle developed pressure (LVDP), left ventricle end-diastolic pressure (LVEDP), +dp/dtmax and-dp/dtmax were also observed. Rhodamine123 Fluorescence fluorescene intensity produced by Rhodamine123 was measured. The percentage recovery of myocardial function of group C were significantly better than control groupB and group D. Compared with group A, Rhodamine123 fluorescence intensity were decreased in group B and group D(p<0.05). CsA can improve the function damage of the immature heart during reperfusion. CsA can prevent the loss ofΔΨm. Taken together, these data can help us to better understand CsA protective effect in immature myocardial ischemia and reperfusion, thus opening new perspectives in immature myocardium protection