DOI: 10.36347/sasjm.2022.v08i02.009

Pages: 106-113

Introduction: The emergence of antibiotic-resistant organisms is a major public health concern, particularly in hospitals and other health care settings. Carbapenem are a group of β- lactam antimicrobial agents with an exceptionally broad spectrum of activity. They are used as a last resort drug against many multi-drug resistant (MDR) microorganisms such as extended-spectrum beta-lactamase (ESBL), Metallo-β- lactamase (MBL), and AmpC β lactamase enzyme-producing gram-negative bacilli. Aim of the study: The aim of this study was to ascertain the prevalence of MBL-producing gram-negative bacilli in a tertiary care hospital. Materials & Methodology: This Cross-Sectional study was carried out in the Department of Microbiology, Chittagong Medical College and Hospital, Chittagong. During the period of July 2015 to June 2016 after approval of the protocol by the ethical review committee of Chittagong Medical College. Total 220 samples were collected from both sexes and different age groups. The specimens were collected and processed according to the standard methodology. Non-molecular methods were used for the detection of Metallo-Beta-Lactamases producing isolates. Results: MBL producing organisms were 100% resistant to Imipenem and Ceftazidime, 98% resistant to Cefotaxime, Cefepime and Amoxicillin-Clavulanic acid, 94% resistant to Ceftriaxone and Ciprofloxacin, 86% resistant to Gentamicin. 84% resistant to Cotrimoxazole and Aztreonam, 78% resistant to Amikacin and Netilmicin, 34% resistant to Piperacillin-Tazobactam and 22% resistant to Colistin. Conclusions: The high rate of Metallo-Beta-Lactamases producing gram-negative bacteria in this study emphasizes the need for active surveillance in the microbiology laboratories for the detection of these resistant strains and also stresses the judicious use of Carbapenems to prevent the spread of the resistant organisms. The non-molecular method was used to detect MBL.