DOI: 10.36347/sajb.2022.v10i07.003

Pages: 154-158

Talinum paniculatum (Jacq.) Gaertn, or known as Javanese Ginseng, refers to one of the medicinal plants that is often used as a raw material for herbal medicine. Its potential is inseparable from the bioactive compounds contained in every part of the plant, especially the saponin. One of the efforts that can be made to increase the saponin content is through the elicitation of callus culture using methyl jasmonate. Therefore, this study aimed to identify the effect of concentration and elicitation time of methyl jasmonate on biomass and saponin production in callus culture. The results showed that variations in concentration and elicitation time had no effect on the decrease of callus biomass when compared to control. The highest callus biomass was produced in the callus which was elicited in MS medium with the addition of a methyl jasmonate concentration of 0.05 mM for 5 days and 0.2 mM for 15 days. In addition, the largest saponin content by stain area (0.563 cm2) and the intensity of the TLC colour intensity (5 out of 5) on the callus were elicited at a methyl jasmonate concentration of 0.15 mM for 15 days. The results of this study indicated that the addition of methyl jasmonate to the callus culture medium could affect the saponin production. In addition, optimal concentration and elicitation time can increase biomass and saponin production in Javanese ginseng callus (T. paniculatum).