DOI: 10.36347/sjams.2013.v01i05.0050

Pages: 552-556

Male infertility is the major multifaceted disorder with several genetic and environmental factors that contribute to the impairment of spermatogenesis.Epidemiological studies reveals that genetic and environmental factors are responsible for the development of male infertility. During spermatogenesis large numbers of extrinsic and intrinsic factors are responsible for the formation of sperm. Later these factors characterize as “ MTHFR and stem cells gene” , both are associated independantly or dependantly in the maintainance of pluripotency during differentiation of germ cell.The aim of the study was to examine the association between MTHFR C677T and Nanog3 , Oct4 & Sox2 gene (s) in etiopathology of male infertility. PCR and RFLP based DNA analysis with selected specific forward/reverse primers. Interestingly, the highest frequency (0.6%) of CT assosited with the high frequency (0.96%) of over-expression for Nanog3observed in male infertility. However, MTHFR mutation is directly linked to the genetict diversity of stem cell gene in the male infertility