DOI: 10.36347/sjams.2013.v01i05.0062

Pages: 600-605

Antimicrobial resistance is not only increasing the healthcare costs, but also the severity and death rates from certain infections that could have been avoided by prudent and rational use of the existing and newer antimicrobial agents. Prudent and rational use of antimicrobial is possible by forming local, national and global wide antibiogram. The present study is undertaken to prepare local antibiogram of Staphylococcus aureus (S. aureus) isolated from pus and to discuss general issues related to antimicrobials use. Total 216 pus sampleswere processed for culture sensitivity testing. Identification of bacteria was done by gram staining. Isolation of the organisms was done by inoculation of samples on agar medium. After 24 hours of inoculation, each organism was identified on the basis of morphology of colony in culture media and biochemical reactions. Antibiotic sensitivity Testing was carried out on Muller Hinton Medium by Disc Diffusion Method following Kirby Bauer method. Out of 216 pus samples were processed, 168 organisms were isolated from pus which includes S.aureus (60), Klebsiellaspp. (36), Pseudomonas (32), Escherichia coli (24),streptococci spp.(16)and others (48). S. aureus is most common organism (27.8 %) isolated from pus. S. aureusis most sensitive to linezolid (86.7%) and gentamicin (86.7 %). An attempt has been made in this study to recognize the S.aureusfrom pus samplesin patients of tertiary care hospital, Surendranagar, Gujarat; to record the antibiogram and probable drug of choice for S.aureus. Considering the antibiotic susceptibility testing, cost, side effects and many other factors; cloxacillin, ampicillin/sulbactam, clindamycin,chloramphenicol and gentamicin should be preferred drugs for S. aureus infection isolated from pus.