DOI: 10.36347/sasjm.2022.v08i08.008

Pages: 555-558

Introduction: In this study, it was aimed to investigate the cytotoxicity and antitumoral activity of casein proteins obtained from bovine milk on colorectal cancer cells (Caco-2). Methods: Milk were fractionated as casein proteins. Antitumor activity levels of different concentrations of lyophilized proteins were measured by MTT viability test on cells and IC50 values were determined. Conclusion: Serial dilutions of casein proteins in milk, starting with 3.200 µg/ml and decreasing, were incubated with Caco-2 cells for 24, 48 and 72 hours. When incubated with bovine casein Caco-2 cells, cell viability: IC50 value found in the 24 hour run 9.172 µg/ml 69.21% at 3.200 µg/ml; IC50 at 48 hours was 8.927 cell viability; 58.5%; The IC50 value at 72 hours was 8.043 µg/ml, and cell viability was found to be 51.91%. It was concluded that bovine casein proteins have limited antiproliferative and anticarcinogenic effects on Caco-2 cancer cell lines.