DOI: 10.36347/sjams.2017.v05i03.057

Pages: 1014-1018

The automated hematology analyzer with complete blood count (CBC) results has replaced the traditional manual or individual assay methods for hematological parameters and the eye count leukocyte differential as the initial screening and detection system for hematological abnormalities in modern hospitals and clinics. Automated method for the estimation of complete blood count is commonly used in routine practice laboratory but many other labs still work on manual procedure for the abnormal automated results as well as health care workers in laboratory can be optimized by doing test on manual microscopic procedure as validation technique for automated method. This study carried out to compare the WBCs by different automated analyzers and manual method. Study carried out in Wad Madani city capital of Gezira State, central Sudan during the period of July to August 2016. The study included 75 healthy individuals selected randomly, 31 of them were males and 44 were females, their age ranges from 20 to 50 years who came for medical examination. Three mls of venous blood were collected from participants in EDTA anticoagulant. The samples were analyzed using haemocytometer, Sysmex XP-300 and Mindray BC-6800.The results showed that repeatability of white blood cells by chamber, mindray and sysmex were (5.1707, 5.0293, 4.9560), (5.8680, 5.8573, 5.8507) and (6.0093, 5.9800, 5.9627) ×10³µl respectively, there was no significant different between the repeated result for manual and mindray but there was significant different by sysmex P.value (0.04).