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Scholars Journal of Applied Medical Sciences | Volume-5 | Issue-01
Identification of parasitic infections in stool samples by different methods: a study emphasizing the use of concentration techniques.
Dr Ritu Garg, Varsha A Singh
Published: Jan. 29, 2017 | 92 74
DOI: 10.36347/sjams.2017.v05i01.032
Pages: 159-161
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Abstract
Parasitic infections remain the major public health issue in the developing countries. The prevalence of intestinal parasitic infections varies according to the geographical locales and also depends upon diagnostic methods used in different laboratories. But most of the laboratories unable to detect parasitic infections by using routine methods like wet mount due to their compromised sensitivity and specificity. Therefore demonstration of parasitic infections from the specimen’s poses a huge challenge to the clinical microbiologists. So by keeping in mind the above facts, a prospective study was conducted over the period of 11 months from January 2015 to November 2015.A total of 100 fresh stool specimens were collected in sterile containers and transported to the Department of Microbiology immediately. A total of 100 stool samples were examined, out of which 45 (45%) samples were positive for ova/cyst by wet mount after concentration method. but the positivity rate by wet mount alone without any concentration method was 36%. The most common identified parasite in our study was that of Entamoeba histolytica (12%) followed by cyst of Giardia (10%), egg of Ascaris (5%),cyst of Cryptosporidium, egg of Ancylostoma, egg of H.nana (4%each), egg of Taenia (3%), cyst of E.coli (2%), egg of Enterobius vermicula is (1%). So there is dire need to use reliable, economical diagnostic methods which can accurately detect parasitic infections and control its spread.